Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Broad bean wilt fabavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.W. Bowyer, 1980.

Nomenclature

Synonyms

catalpa chlorotic leaf spot virus (Schmelzer, 1970), tropaeolum ringspot virus (Cook and Gibbs, 1971), nasturtium ringspot virus, Ringmosaikvirus der Kapuzinerkresse (Frowd and Tomlinson, 1972), petunia ringspot virus (Hull and Plaskitt, 1973; 1974), P.O. pea streak virus, parsley virus 3.

Acronym

BBWV, BBWV-1, BBWV-2

ICTV decimal code

18.0.2.0.001

Host range and symptoms

Natural host range and symptoms

Symptoms vary seasonally.
• Vicia faba, Pisum sativum - vein clearing, mottling and necrosis of shoot apex, plant wilts, mottled, malformed and stunted.
• Spinacia oleracea - wilting, shoot die-back, leaves mottled.
• Petroselinum crispum - symptomless.
• Tropaeolum majus - mosaic.
• Petunia × hybrida - line-ring spots and oak leaf patterns.
• Plantago lanceolata - mottling, ringspots.

Transmission

Transmitted by a vector; an insect; Acyrthosiphon pisum, Aphis craccivora, A. faba, A. nasturtii, Macrosiphum euphorbiae, M. solanifolii, M. persicae; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; not transmitted by seed.

Geographical distribution

Spreads in the African region, the Eurasian region, the Middle East, the North American region, and the Pacific region; Australia and China. Found, but with no evidence of spread, in Argentina.

Experimental host range

Many (>9) families susceptible. Experimentally infected plants mostly show necrotic local lesions, ringspots, mosaic and systemic necrosis.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; tip epinasty, leaves malformed and mottled.
• Spinacia oleracea - systemic wilt, necrosis and die-back.
• Vicia faba - apical necrosis, wilting and mosaic.
• Vigna unguiculata cv. Blackeye - red or chlorotic local lesions; systemic mosaic and malformation.
• Datura stramonium - coalescent, necrotic local lesions; systemic ringspots and line patterns.
• Nicotiana × edwardsonii - necrotic local lesions; systemic necrosis.
• N. glutinosa - systemic mosaic with some ringspots.
• N. tabacum cv. White Burley - large ringspots; systemic leaf chlorosis.
• Petunia × hybrida - systemic line and oak leaf patterns and ringspots.

Maintenance and propagation hosts

Chenopodium quinoa, Pisum sativum, Vicia faba, Nicotiana × edwardsonii.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L), Phaseolus vulgaris (Moroccan isolate only), Vigna unguiculata cv. Blackeye (L), Datura stramonium (L).

Susceptible host species

• Antirrhinum majus
• Beta vulgaris
• Brassica campestris
• Brassica campestris ssp. rapa
• Calendula officinalis
• Capsella bursa-pastoris
• Capsicum frutescens
• Chenopodium amaranticolor
• Chenopodium hybridum
• Chenopodium quinoa
• Cucumis sativus
• Cyamopsis tetragonoloba
• Datura metel
• Datura stramonium
• Glycine max
• Gomphrena globosa
• Lactuca sativa
• Lathyrus odoratus
• Lupinus angustifolius
• Matthiola incana
• Nicandra physalodes
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Nicotiana × edwardsonii
• Ocimum basilicum
• Petroselinum crispum
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Physalis peruviana
• Pisum sativum
• Plantago lanceolata
• Solanum nigrum
• Spinacia oleracea
• Tropaeolum majus
• Vicia faba
• Vigna unguiculata

Insusceptible host species

• Brassica oleracea var. botrytis
• Cajanus cajan
• Capsicum annuum
• Chenopodium amaranticolor
• Cucumis melo
• Helianthus annuus
• Lycopersicon esculentum
• Lycopersicon pimpinellifolium
• Phaseolus lunatus
• Raphanus sativus
• Solanum tuberosum
• Sonchus oleraceus
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (5/5)
• Compositae (2/5)
• Cruciferae (4/6)
• Cucurbitaceae (1/2)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (8/10)
• Plantaginaceae (1/1)
• Scrophulariaceae (1 /1)
• Solanaceae (15/19)
• Tropaeolaceae (1/1)
• Umbelliferae (1/1)

Families containing insusceptible hosts

• Chenopodiaceae (1/5)
• Compositae (3/5)
• Cruciferae (2/6)
• Cucurbitaceae (1/2)
• Leguminosae-Papilionoideae (2/10)
• Solanaceae (4/19)

Sources of host-range data

Gracia and Feldman (1976); Schmelzer and Stahl (1977).

Physical and biochemical properties

Properties of particles in sap

TIP: 55-60 °C. LIV: 3-4 days. DEP: log₁₀ minus 4-5. Leaf sap contains many virions.

Purification method

Doel (1975).

Particle morphology

Virions isometric; not enveloped; 25 nm in diameter, or 28-32 nm in diameter (for parsley virus 3); angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 126 S (B); of the other(s) 100 S (M), or 63 S (T). Density in the presence of 2.34 w/v Igepon T₇₃ 1.39 g cm^-3 in CsCl (M), or 1.44 g cm^-3 in CsCl (B). A₂₆₀/A₂₈₀ ratio 1.69 (M), or 1.79 (B).

Biochemical properties

Virions contain 33 % nucleic acid (B), or 22 % nucleic acid (M), or 0 % nucleic acid (T); 67 % protein (B), or 78 % protein (M), or 100 % protein (T); 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 10.8 kb. Genome of two parts; largest (or only) genome part the larger 6.3 kb; the 2nd largest 4.5 kb. Base composition 26 % G; 30 % A; 17.5 % C; 26.5 % U. Infectivity retained when deproteinised with phenol or detergent.

Features of proteins

Virion protein(s) two; M_r of the larger 42000. M_r of 2nd largest 26000. Method of preparation: Doel (1975); Brown and Hull (1973).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in mesophyll and epidermis; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm (Russo et al., 1975), or amorphous X-bodies (Russo and Martelli, 1975); they contain virions (the inclusions are unlike those induced by members of the comovirus group, yet are similar to those induced by some tymoviruses; Edwardson and Christie, 1986).

Taxonomy and relationships

Fabavirus: Comoviridae (the Type species of the genus)

Virus(es) with serologically related virions

All isolates are serologically closely related and indistinguishable.

Virus(es) with serologically unrelated virions

Four comoviruses.

Comments and References

References

• Brown, F. and Hull, R. (1973). J. gen. Virol. 20: 43.
• Cook, S.M. and Gibbs, A.J. (1971). Rep. Rothamsted Exp. Stn for 1970, p. 124.
• Doel, T.R. (1975). J. gen. Virol. 26: 95.
• Edwardson, J.R. and Christie, R.G. (1986). Fla Agric. Res. Stn Monog. No. 14, p. 95.
• Frowd, J.A. and Tomlinson, J.A. (1972). Ann. appl. Biol. 72: 189.
• Govier, D.A. (1988). Ann. appl. Biol. 113: 287.
• Govier, D.A. (1988). Ann. appl. Biol. 113: 287.
• Gracia, O. and Feldman, J.M. (1976). Phytopath. Z. 85: 227.
• Harrison, B.D. (1976). Rep. Scottish Hort. Res. Inst. 1976.
• Hull, R. and Plaskitt, A. (1973/74). Intervirology 2: 352.
• Lockhart, B.E.L. and Fischer, H.U. (1977). Phytopath. Z. 88: 209.
• Russo, M. and Martelli, G.P. (1975). J. Submicrosc. Cytol. 7: 335.
• Sahambi, H.S., Milne, R.G., Cook, S.M., Gibbs, A.J. and Woods, R.D. (1973). Phytopath. Z. 76: 158.
• Schmelzer, K. (1970). Phytopath. Z. 67: 285.
• Schmelzer, K. and Stahl, P. (1977). Zbl. Bakt. ParasitKde. Abt. 2, 132: 123.
• Schroeder, W.T. and Provvidenti, R. (1970). Phytopathology 60: 1405.
• Shukla, D.D., Teakle, D.S. and Gough, K.H. (1980). Plant Dis. 64: 802.
• Stubbs, L.L. (1947). J. Dep. Agric. Vic. 46: 323.
• Stubbs, L.L. (1960). Aust. J. agric. Res. 11: 734.
• Taylor, R.H. and Stubbs, L.L. (1968). Aust. J. biol. Sci. 21: 929.
• Taylor, R.H. and Stubbs, L.L. (1972). CMI/AAB Descr. Pl. Viruses No. 81, 4 pp.
• Tian, W.H., Wang, X.F., Pei, M.Y. and Xie, D.Z. (1982). Acta Phytopath. Sin. 9: 153.
• Uyemoto, J.K. and Provvidenti, R. (1974). Phytopathology 64: 1547.
• Xi, Z.X., Xu, S.H. and Mang, K.Q. (1982). Acta Phytopath. Sin. 12: 38.
• Xu, Z.G., Cockbain, A.J., Woods, R.D. and Govier, D.A. (1988). Ann. appl. Biol. 113: 287.
• Xu, Z.G., Pu, Z.C., Cao, Q., Fang, Z.D. and Cockbain, A.J. (1985). J. Nanjing Agric. Univ. 1985, pp. 42-48.

Illustrations

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 16^th January 1997.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.